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產(chǎn)品詳情
| 名稱： | anti- GAPDH antibody |
|---|---|
| 貨號： | FNab03343 |
| 適應(yīng)種屬： | 人源,大鼠,小鼠 |
| 規(guī)格： | 100ug(微克) |
| 狀態(tài)： | 液體 |
| 純化方法： | 蛋白A/G柱純化 |
| 純度： | 經(jīng)SDS-PAGE分析,純度大于95% |
| 宿主： | 小鼠 |
| 亞型： | IgG1 |
| 存儲： | 用添加了0.02%疊氮化鈉和50%甘油的PBS溶解,PH值7.3,可在-20℃下保存12個月（避免反復(fù)凍融） |
| 免疫原： | glyceraldehyde-3-phosphate dehydrogenase |
| 免疫原別稱： | G3PD antibody, GAPD antibody, GAPDH antibody |
| 分子量： | 36 kDa |
| 驗(yàn)證應(yīng)用： | ELISA, IHC, WB, IF, FC, IP |
| 建議稀釋比例： | WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200 |
| UniProt ID： | P04406 |
| 克隆性： | 單克隆 |
| 背景介紹： | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition i |
| 圖像： |  Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab03343(GAPDH Antibody) at dilution of 1:100。  IP Result of anti-GAPDH (IP: FNab03343, 4ug; Detection: FNab03343 1:3000) with Hela cells lysate 3000ug。  HeLa cells were subjected to SDS PAGE followed by western blot with FNab03343(GAPDH antibody) at dilution of 1:10000。 |
