服務(wù)熱線
15623758226
聯(lián)系方式
- 手機(jī)：15623758226
- 電話：027-65524773
- Q Q：3038053592
- 郵箱：3038053592@qq.com
- 地址：武漢東湖新技術(shù)開發(fā)區(qū)高新二路388號武漢光谷國際生物醫(yī)藥企業(yè)加速器1.2期C22棟一層、二層
發(fā)布時間：2024-04-16 15:26?? 咨詢量：
聯(lián)系客服 027-65524773
資料下載sales2@fn-test.com
產(chǎn)品詳情
| 名稱： | anti- beta actin antibody |
|---|---|
| 貨號： | FNab00869 |
| 適應(yīng)種屬： | 人源,大鼠,小鼠 |
| 規(guī)格： | 100ug(微克) |
| 狀態(tài)： | 液體 |
| 純化方法： | 免疫親和純化 |
| 純度： | 經(jīng)SDS-PAGE分析,純度大于95% |
| 宿主： | 兔 |
| 亞型： | lgG |
| 存儲： | 用添加了0.02%疊氮化鈉和50%甘油的PBS溶解,PH值7.3,可在-20℃下保存12個月（避免反復(fù)凍融） |
| 免疫原： | actin, beta |
| 免疫原別稱： | ACTB antibody, Actin antibody, actin antibody, beta antibody, Actin antibody, cytoplasmic 1 antibody, B actin antibody, Beta actin antibody, F actin antibody, β actin antibody |
| 分子量： | 42 kDa |
| 驗證應(yīng)用： | ELISA, IF, WB, IHC, IP |
| 建議稀釋比例： | WB: 1:500-1:5000; IHC: 1:20-1:500; IF: 1:50-1:500;IP: 1:500-1:2000 |
| UniProt ID： | P60709 |
| 克隆性： | 多克隆 |
| 背景介紹： | Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. |
| 圖像： |  Immunohistochemistry of paraffin-embedded human heart using FNab00869(ACTB antibody) at dilution of 1:100。  Various lysates were subjected to SDS PAGE followed by western blot with FNab00869(beta actin Antibody) at dilution of 1:2000。 |
